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Chemistry of Biomacromolecules

Professor Emeritus
Address: Circuito Exterior s/n, Ciudad Universitaria.District Coyoacán. ZIP Code 04510 Mexico City. Mexico

Telephone: +52 (55) 56-24-56-67
Fax: +52 (55) 56-16-22-17

 

 

 

ACADEMIC SUMMARY

Ph.D. California Institute of Technology (Caltech), Biology Division. 
1943-1946.

Research Fellow. California Institute of Technology (Caltech), Biology Division
1947-1950.

Biochemist. Syntex Laboratories, México. January 1952 to June 1954.

Full-time Researcher. UNAM. Institute of Chemistry, with a Rockefeller Foundation grant,
July 1954 to January 1955. He was founder of the Biochemistry Lab at the Institute of Chemistry.

UNAM. Institute of Chemistry. Full-time Researcher, with University’s funds since February, 1955.

Max-Planck Institut für Zellchemie. Munich, Germany. Sabbatical Leave.
August-December 1958. Research on the biosynthesis of rubber in hevea, employing isopentenyl-C14 pyrophosphate as precursor, collaborating with Feodor Lynen, Director of that Institute and winner of the Nobel Prize in 1964. Later, he visited the Institute for short periods, in 1964, 1972 and 1976.


 
 

RESEARCH TOPICS

Experiments on sucrose formation by potato tubers influenced by temperature.

Carbohydrate metabolism in potato tubers. Doctoral dissertation to obtain Ph.D. degree. 1943-1946. Biology Division, California Institute of Technology.

Biochemistry of rubber formation in Guayule. I. Rubber formation in seedlings.

Biosynthesis of guayule employing cultured tissues and plants; function of acetate-14C. 1947-1950 (three articles). California Institute of Technology, Pasadena, Calif. USA. It was demonstrated that the acetate is the precursor of this cis poliisoprenoide, as occurs in most isoprenoids.

 

Studies of methylglyoxal and glyoxalase

Study and isolation of enzymes from different plants: glyoxalase, D-aminoacid oxidase, lactate dehydrogenase, isomerase, aldolase, phosphorilase, etc., their purification and kinetic, determination of Michaelis constant and pH effect, part of this work was performed with a Warburg apparatus, the first equipment acquired for the incipient Biochemistry lab, and subsequently, through different methodologies. Enzymes were the prominent topic of study in those years, 1954-1964.

 

Trimethylsilyl sugars at various temperatures

Demonstration of the pentose phosphate pathway and study of hevea latex by a novel methodology. Elimination of the enzymatic phosphate from phosphor sugars formed by this pathway, after salinization of sugars and demonstration of their presence by their subsequent separation using gas liquid chromatography (GLC), a novel methodology in those times and hardly ever used for enzymatic studies. 14C Glucose-1 was employed to demonstrate the presence of oxide-reduction reactions at the pentose-phosphate cycle, keeping the free 14CO2. These reactions are coupled in the hevea latex to the biosynthesis of rubber (cis polisoprenoide); predominant product of this material metabolism. It was proposed that NADPH formed through this pathway is employed in the latex to reduce the 3-OH3-methyl glutaryl CoA to mevalonic acid. This same study was repeated with the higueronia latex, this plant produced the gutapercha trans-polyisoprenoid, thus demonstrating the presence of this metabolic pathway and its participation in the biosynthesis of trans-polyisoprenoid.

In the mid-eighties Dr. Arreguín pioneer studies on the biochemistry of marine organisms (not frequently studied in Mexico despite its great marine coast). The isolation and characterization of proteins and enzymes, and in an outstanding way, he has worked in the isolation and characterization of lectines from marine and freshwater organisms, 1987-2004.

Studies of the elucidation of the three-dimensional structure of proteins by X-ray diffraction are under way.

 

RECENT PUBLICATIONS

  • PFA, A Novel Mollusk Agglutinin, is Structurally Related to the Ribosome-Inactivating Protein Superfamily; R. Arreguín-Espinosa, B. Fenton, E. Vázquez-Contreras, B. Arreguín y E. García-Hernández; Archives Biochem. Biophys. 394, 151-155 (2001).
  • Purification and Structural Characterization of Lectins from the Cnidarian bunodeopsis; Bertha Fenton-Navarro, Barbarín Arreguín L., Enrique García-Hernández, Edgar Heimer, Manuel B. Aguilar, Claudia Rodríguez-A., Roberto Arreguín-Espinosa; Toxicon 42, 525-532 (2003).
  • Purification and Characterization of a Liver Lectins from a Lizard, Sceloporus spinosus. Fenton N. B, Arreguín, B., Méndez C. F. and Arreguín E. R. Prep. Biochem. Biotech. 34: 153-168, 2004.
  • Bertha Fenton, Barbarín Arreguin-Lozano, Enrique García Hernández, Edgar Vázquez-Contreras, Norma Sánchez-Sánchez, Victor Farías-Rodriguéz, G. Partida Hernández, Edgar Zenteno and Roberto Arreguín-Espinosa (2006). Lectins from marine sponge Spheciospongia vesparia. Purification and primary Structures. Protein Science 15, Suppl. 1, August 2006, pag. 268.
  • Vanessa Cabra, Barbarin Arreguin and Roberto Arreguín-Espinosa (2007). Effect of sulfhydryl groups on aggregation and oligomerization process of ?-zeins Protein Science 16, Suppl. 1, July 2007, pag. 276.
  • Beltrán Robles, A., Cabra Ledesma, V.; Arreguín, B.; Arreguín-Espinosa, R.* A lactose specific lectin from the sponge Neopretosia subtriangularis: Purification, characterization, N-terminal sequences alignment and agglutinating activity on Leishmania promastigotes. Comp. Biochem. Phys. B, 2010, 175, 221-226. 




 

 
 
 





 

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